Aloin Induces Gastric Cancer Cell Apoptosis via the miR-5683/HMGB1 Signal Axis

Background: Recent studies conducted by us indicate that aloin (ALO) could promote gastric cancer (GC) cell apoptosis, but the underlying mechanism remains unclear. The high mobility group box 1 (HMGB1) has been reported to regulate proliferation, and migration of several types. Bioinformatics analysis suggests a possible targeted regulatory relationship between miR5683 HMGB1. Purpose: Herein, we aimed investigate role miR-5683/HMGB1 axis in ALO-induced GC apoptosis. Methods: expression levels miR-5683 HMGB1 tissue adjacent normal were investigated using bioinformatics analysis, their expressions at cellular determined reverse transcription quantitative real-time polymerase chain reaction western blotting (WB) assays. interaction was predicted TargetScan database verified dual-luciferase assay. Cell viability apoptosis assessed counting kit 8 (CCK-8) assay, 4′,6-diamidino-2-phenylindole (DAPI) staining, flow cytometry. WB used examine apoptosis-related proteins Results: Our data showed ALO promoted downregulated HMGB1, upregulated expression, assay confirmed miR-5683. Moreover, mimics enhanced inhibitory effects on However, inhibitors opposite effects. Conclusion: suggested promotes through axis.